The affinity-labelling of cathepsin s with peptidyl diazomethyl ketones

The inactivation of the cysteinyl exopeptidases cathepsin H and C by affinity-labelling reagents.

An attempt has been made to extend to the cysteinyl exopeptidases cathepsins H and C affinity-labelling approaches shown to be effective with cysteinyl endopeptidases such as cathepsins B and L and the calcium-activated proteinase. This involved the preparation of amino acid and dipeptide derivatives with unblocked N-termini to satisfy the aminopeptidase and dipeptidyl aminopeptidase characteri...

PREPARATION AND EVALUATION OF AOMKS FOR CATHEPSIN B IMAGING PREPARATION AND EVALUATION OF PEPTIDYL ACYLOXYMETHYL KETONES FOR CATHEPSIN B IMAGING By PATRICIA E. EDEM, B.Sc. (Hons.)

Affinity-labelling of the thyrotropin receptor

Thyrotropin (TSH) has been coupled to the photoactive heterobifunctional reagent N-hydroxysuccinimidyl 4-azidobenzoate (HSAB) and the properties of the product (HSAB-TSH) investigated. Preparations of HSAB-TSH containing two molecules of HSAB per molecule of TSH were used in most experiments and these preparations retained about 40% of the original receptor-binding activity of the TSH. HSAB-TSH...

Affinity labelling of 5'-nucleotidases with 5'-p-fluorosulphonylbenzoyladenosine.

5'-Nucleotidases play an important role in the metabolism of nucleosides; for example, the hydrolysis of AMP generates adenosine, which can modulate a variety of cellular functions. We have used the membrane-bound AMPase from chicken gizzard and a secreted form of these enzymes to analyse their modification by the substrate analogue 5'-p-fluorosulphonylbenzoyladenosine (5'-FSBA). 5'-FSBA irreve...

determinant of the hankel matrix with binomial entries

abstract in this thesis at first we comput the determinant of hankel matrix with enteries a_k (x)=?_(m=0)^k??((2k+2-m)¦(k-m)) x^m ? by using a new operator, ? and by writing and solving differential equation of order two at points x=2 and x=-2 . also we show that this determinant under k-binomial transformation is invariant.